Exploiting Human CD34+ Stem Cell–conditioned Medium for Tissue Repair

Exploiting Human CD34+ Stem Cell–conditioned Medium for Tissue Repair

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble f...

Human Pluripotent Stem Cells Differentiated in Fully Defined Medium Generate Hematopoietic CD34+ and CD34− Progenitors with Distinct Characteristics

BACKGROUND Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In particular, the use of a fully defined medium (FDM) would avoid biases induced by unidentified factors contained in serum, and would also allow key molecular mediators involved in such a process to be identified. Our goal was to induce in ...

Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb.

RATIONALE Paracrine secretions seem to mediate therapeutic effects of human CD34+ stem cells locally transplanted in patients with myocardial and critical limb ischemia and in animal models. Earlier, we had discovered that paracrine secretion from human CD34+ cells contains proangiogenic, membrane-bound nanovesicles called exosomes (CD34Exo). OBJECTIVE Here, we investigated the mechanisms of ...

Comparative study on differentiation characteristics of CD34-negative and CD34-positive human adipose tissue-derived stem cell subpopulations.

Adult Mesenchymal Stem Cells for Tissue Engineering

The identification of multipotential mesenchymal stem cells (MSCs) derived from adult human tissues such as bone marrow and connective tissues has provided exciting prospects for cell-based tissue engineering and regeneration. This review article focuses on the biology of MSCs, their differentiation potentials in vitro and in vivo, and their application in tissue engineering...